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Proteomics and modified peptides platform (P3M)

Joint Service Unit PLBS (Platforms Lille in Biology & Health) – UMS 2014 – US41

Plateforme d'analyses P3M

Presentation

The Plateforme d’analyse Protéomique et de Protéines Modifiés (P3M)  is a technical platform of the Institute Pasteur of Lille. P3M offers expertise ranging from the identification of proteomes by nanoLC-MSMS and the use of databases or de novo analysis, to their quantification with or without labeling. The available equipment also allows the study of post-translational modifications (PTM) of small sizes with or without enrichment, as well as the characterization of protein maturation events.

PTM analysis of a protein can be performed at the peptide level, but also by mass analysis of the entire protein by LC-MS. P3M also has the native mass know-how for the analysis of interactions between proteins, ligands or nucleic acids. P3M belongs to the service unit PLBS – UMS 2014 – US 41 (https://ums-plbs.univ-lille.fr/).

Highlights

  • January 2020 : P3M integrates the service unit PLBS.
  • Jume 2020 : Developpement of the protein’s Thermal Profiling by TMT quantification.
  • September 2020 : AS Lacoste recruitment.
  • January 2021 : the collaboration between P3M and PAGés (Plateforme d’Analyse des Glycoconjugués) is certified by the Infrastructure in BioHealth/Agriculture label (GIS IBiSA – “Infrastructure en Biologie Sante et Agronomie“).

Transversal projects

  H2020-SC1-BHC. Acronyme : FAIR

JC Sirard, Center of Infection and Immunity of Lille : Flagellin Aerosol Therapy as an Immunomodulatory Adjunct to the Antibiotic Treatment of Drug-Resistant Bacterial Pneumonia.

  Glycoprotéomic project

Collaboration with the PAGés for the characterization and quantification of antibody’s glycopeptid.

Members

Jean-Michel SALIOU
Research engineer IPL, group leader

Anne-Sophie LACOSTE
Research engineer UL

Publications

Rivera-Millot A, Slupek S, Chatagnon J, Roy G, Saliou JM, Billon G, Alaimo V, Hot D, Salomé-Desnoulez S, Locht C, Antoine R, Jacob-Dubuisson F.
Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon.
Commun Biol. 2021 Jan 8;4(1):46. doi: 10.1038/s42003-020-01580-2. PMID: 33420409.

Trzaska C, Amand S, Bailly C, Leroy C, Marchand V, Duvernois-Berthet E, Saliou JM, Benhabiles H, Werkmeister E, Chassat T, Guilbert R, Hannebique D, Mouray A, Copin MC, Moreau PA, Adriaenssens E, Kulozik A, Westhof E, Tulasne D, Motorin Y, Rebuffat S, Lejeune F.
2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations.
Nat Commun. 2020 Mar 20;11(1):1509. doi: 10.1038/s41467-020-15140-z. PMID: 32198346; PMCID: PMC7083880.

Nagnan-Le Meillour P, Descamps A, Le Danvic C, Grandmougin M, Saliou JM, Klopp C, Milhes M, Bompard C, Chesneau D, Poissenot K, Keller M.
Identification of potential chemosignals in the European water vole Arvicola terrestris.
Sci Rep. 2019 Dec 5;9(1):18378. doi: 10.1038/s41598-019-54935-z. PMID: 31804568; PMCID: PMC6895148.

Lesage KM, Huot L, Mouveaux T, Courjol F, Saliou JM, Gissot M.  
Cooperative binding of ApiAP2 transcription factors is crucial for the expression of virulence genes in Toxoplasma gondii.
Nucleic Acids Res. 2018 Jul 6;46(12):6057-6068. doi: 10.1093/nar/gky373. PMID: 29788176; PMCID: PMC6159514.

Montpellier C, Wychowski C, Sayed IM, Meunier JC, Saliou JM, Ankavay M, Bull A, Pillez A, Abravanel F, Helle F, Brochot E, Drobecq H, Farhat R, Aliouat-Denis CM, Haddad JG, Izopet J, Meuleman P, Goffard A, Dubuisson J, Cocquerel L.  
Hepatitis E Virus Lifecycle and Identification of 3 Forms of the ORF2 Capsid Protein.
Gastroenterology. 2018 Jan;154(1):211-223.e8. doi: 10.1053/j.gastro.2017.09.020. Epub 2017 Sep 25. PMID: 28958858.


Keywords

Proteomics ; Post traductional modification ; Glycoproteomics ; Native mass

Team contact

Jean-Michel Saliou
Research engineer, group leader

jean-michel.saliou@pasteur-lille.fr
03 20 87 71 76