HIGH SECURITY LABORATORY Institut Pasteur de Lille Responsable Jean-Pierre DECAVEL
00 33 3 20 87 79 50
Group members DECAVEL Jean-Pierre | LEGRAND Damien | VANDENABEELE Nicolas
The study of the emergent diseases such as SARS, bird flu, or of diseases in outbreak like tuberculosis, require conditions of
safety reinforced both for the worker and the environment.
The High Security Laboratory L.H.S (NSB 3-4) of the Institut Pasteur de Lille has the role to fulfill the legal requirements and techniques of handling the GMO’s or pathogenic dangerous for the environment (up to level 4 in BCS 3 and Gloves box) or the public health.
The handling of the man or animal diseases always required specific working conditions.
The concept of containment physical of the laboratories for the handling of the micro-organisms of levels 1, 2, 3 and 4 appeared within the U.S.A in the Seventies. This concept was created to protect the environment during handling and the study from the GMO's research activities or analyses in microbiology. The study of the emergent diseases such as SARS, bird flu (H5N1), highly pathogenic, or of the diseases such as tuberculosis, but also the handling of GMO's request an optimal safety and security level both for the protection of the worker and of the environment.
Work on biological agent in the LHS is carried out with respect to the French and European legislations , but also of the reference on the GMO' S, or those of dangerous pathogenic agents for the environment and the public health, it should be noted that the French regulation is rather recent (first texts were adopted in the years 1990) and rises primarily from the transposition of European directives.
The decree of July 18th 1994 modified in 1997 and 1998 fixes the list of the pathogenic biological agents. The classification mentioned in these lists is delicate exploitation because the associated biological risk is very lovoad and is evolving progressively with new knowledge. It is then necessary to consult lists coming from foreign countries, and thus with the assistance of professional people to establish risk assessment based on the various criteria of classifications. : severity of the disease, dissemination in the environment; existence of preventive or curative treatments.
The decree of July 16th, 2007 prevention, in particular of containment, where the workers are likely to be exposed to pathogenic biological agents.
Lastly, the law 92-654 of July 13th 1992 and the texts of its application refer to the control of the use and the dissemination
of the GMO’S. A good training, completed by a high level of knowledge are requested from the personnel working the LHS. Buildings,facilities and equipment of the laboratory of high safety are devoted to the teams of the Calmette Site, (Institut Pasteur de lille and Institut de Biologie de Lille) including the university or the CHRU.
The units carried out in cast solid polyester panels guarantee an absolute level of sealing. The insulation of the cells (laboratories) allows the reception of at least eight to ten different research themes, getting for this structure an original character compared to other laboratories of this type designed to fulfill the requirements for only one pathogen.
The laboratory allows the implementation of all the techniques, energy of the cellular culture to animal handling, the viral production of parasites, including the emergent disease such as SARS or H5N1 strains.
Safety and security, versatility and evolutionarity thus guided the design of this laboratory for which equipment was or is still specifically designed.
The laboratory, installed on three floors :
A facility which supports the ert treatment. This one is sterilized by filtration, inlet and outlet, on a high efficiency filter ULPA.
9 working areas or independent laboratories located in the ground floor. These cells (laboratories) can host 25 researchers and technicians simultaneously, equipped with 14 biological safety cabinet BSC II and 6 insulators for animals housing.
1 Hight Security Zone (level 4) equipped with 1 Biologcal safety cabinet BSC III and 3 High containment insulators (Gloves boxes).
1 zone equipped with three -80° freezers.
The LHS waste are decontaminated by one effluent decontamination system and one autoclave.
The laboratory of high safety is placed under the responsibility of Jean-Pierre De CAVEL assisted by several technicians.
JP DE CAVEL (Other Field )
(a) French Expert in 6 th Framework Programme on Research, Technological Development and Demostration Coodination Action: BIOSAFETY- EUROPE.
(b) Expert for The Bureau d’Expérimentation Animale de l’INSERM.
(c ) Biosasety and Biosecurity Expert for AFSSAPS.